Preparation of medium S2T2 | Ca(NO3) 4H2O | 100 mg | | KNO3 | 100 mg | | MgSO4 7H2O | 30 mg | | K2HPO4 | 40 mg | | FeCl3.6H2O | 1 ml | | Soil extract 2 | 40 ml | | Sphagnum extract 3 | 20 ml | | Distilled Water | 1000 ml |
1 Soil extract:
Weight 200 g of garden soil without great humus content, fertilizers or other protective agents. Add 1 liter of deionized water, and sterilize by heating for 1 hour.
Separate the decanted extract from particules by centrifugation. Fill into small containers of stock solution, autoclave for 1 hour at 1 atmosphere in three consecutive days. Store in the refrigerator.
2 Sphagnum extract:
Weight 50 g of dried Sphagnum, add 1.5 l of deionized water and sterilize by heating for 1 hour. Decant the extract. Fill into small containers and autoclave for 1 h at one atmosphere in three consecutive days. Store in the refrigerator.
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