Medium: 

A-LCol

 Composition: 

Preparation of solidified medium LCol

 
Ca(NO3) 4H2O40 mg 
KNO3100 mg
MgSO4 7H2O30 mg
K2HPO440 mg
Oligo I0.5 ml
Oligo II0.5 ml
Distilled Water1000 ml

Add 1.5% agar before autoclaving.

 

Composition of the Oligo I solution 1

CuSO4 5H2O0.3 mg
(NH4)6Mo7O244H2O0.6 mg
ZnSO4 7H2O0.6 mg
CoCl2 6H2O0.6 mg
Mn(NO3)2 4H2O0.6 mg
C6H8O7 H2O0.6 mg
H3Bo30.6 mg
Distilled water10 ml

Composition of the Oligo II solution 1

C6H3O7Fe 5H2O16.25 mg
FeSO4 7H2O6.25 mg
FeCl3 6H2O6.25 mg
Distilled water10 ml

Autoclave 20 minutes at one atmosphere and store in the refrigerator.